Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
KPNA6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
KPNA6 antibody was raised against a 14 amino acid synthetic peptide near the internal of KPNA6.
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 60 kDa band observed in human skeletal muscle lysates (calculated MW of 60.0 kDa according to NP_036448.1). Recommended concentration:0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human adrenal gland shows staining of cytoplasm and some nuclear membrane (nuclear pores). Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.