ANKK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ANKK1 antibody was raised against a 15 amino acid synthetic peptide near the internal region of ANKK1.
ANKK1
Reactivity: Mouse, Rat
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 555
Application Notes
ELISA: Antibody detection limit dilution 1:128000. Approximately 85 kDa band observed in human brain (Substantia nigra and amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ANKK1
(Ankyrin Repeat and Protein Kinase Domain-Containing Protein 1 (ANKK1))