PCK1 antibody

Catalog No. ABIN249350

Product Details anti-PCK1 Antibody

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Mouse, Zea mays, Green bean, Miscanthus giganteus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): No cross-reactivity with: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 73 / 78 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 73 | 78 kDa.

Comment:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-PCK1 antibody (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PEPCK (PCK1 Products)

Synonyms: PEPCK-C antibody, PEPCK1 antibody, PEPCKC antibody, PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, 1810010O14Rik antibody, 9130022B02Rik antibody, cb856 antibody, cb924 antibody, fj93h11 antibody, zgc:63869 antibody, wu:fc51c05 antibody, wu:fj93h11 antibody, pepck-c antibody, pepck1 antibody, pepckc antibody, PCK1 antibody, PPCK1 antibody, AI265463 antibody, Pck-1 antibody, GTP antibody, PCK antibody, Pepck antibody, RATPEPCK antibody, Ppc1C antibody, PHOSPHOENOLPYRUVATE CARBOXYKINASE antibody, T28I19.150 antibody, T28I19_150 antibody, phosphoenolpyruvate carboxykinase 1 antibody, 143299_at antibody, CG10924 antibody, CG17725 antibody, Dmel\\CG17725 antibody, Dromel_CG17725_FBtr0086701_pepck_mORF antibody, dPEPCK antibody, pepck antibody, PEPC antibody, PEPCase antibody, ppc antibody, phosphoenolpyruvate carboxykinase 1 antibody, phosphoenolpyruvate carboxykinase 2, mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, phosphoenolpyruvate carboxykinase 1 (soluble) antibody, phosphoenolpyruvate carboxykinase 1 S homeolog antibody, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody, phosphoenolpyruvate carboxykinase 1, cytosolic antibody, phosphoenolpyruvate carboxylase 7 antibody, Phosphoenolpyruvate carboxykinase antibody, phosphoenolpyruvate carboxylase antibody, PCK1 antibody, PCK2 antibody, Pck2 antibody, pck1 antibody, pck1.S antibody, LOC100634531 antibody, Pck1 antibody, pep7 antibody, Pepck antibody, LOC107777405 antibody

Background: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2. Both genes are nuclear, located on chromosome 4 and 5 respectively (in Arabidopsis). The protein products, PEPCK1 and PEPCK2, are both highly conserved in the model plant, Arabidopsis thaliana.

Molecular Weight: expected: 73 kDa, apparent: 78 kDa

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis