Rabbit anti-Monkey IgA (Fc Region) Antibody (FITC)

Catalog No. ABIN2622424

Product Details

Target: IgA

Binding Specificity: Fc Region

Reactivity: Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Monkey IgA Fc specific.

Purification: Purified IgG

Immunogen: Pools of purified IgA isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, ICC, IF, IHC, IHC-Fr

Usage: The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against monkey serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgA used as immunogens. It does not react with IgG, IgG/Fab fragments and IgM or any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Monkey

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody