Goat anti-Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c (Fc Region) Antibody (FITC)
ELISA, ICC, IHC, IF, IHC (fro)
Polyclonal
FITC
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- Target
- IgA + IgG1 + IgG2a + IgG2b + IgG2c
- Binding Specificity
- Fc Region
- Reactivity
- Rat
- Host
- Goat
- Clonality
- Polyclonal
- Conjugate
- FITC
- Application
- ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Rat IgG, IgA and IgM, Fc specific.
- Purification
- Purified IgG
- Immunogen
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Purified polyclonal IgG and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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- Application Notes
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Approved: ELISA, ICC, IF, IHC, IHC-Fr
Usage: The reactivity of the antiserum is directed to the Fc subunit of the major immunoglobulin isotypes (classes and subclasses) which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bound to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2. No preservative added.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- IgA + IgG1 + IgG2a + IgG2b + IgG2c
- Background
- This antibody detects: IgA + IgG1 + IgG2a + IgG2b + IgG2c Fc
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