Goat anti-Rabbit IgG (Whole Molecule) Antibody (PE)

Catalog No. ABIN2622737

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Whole Molecule

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Specificity: Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Purification: Immunoaffinity purified

Immunogen: Rabbit IgG whole molecule.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IF (1:100 - 1:250)

Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10 x10⁶ in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody