Goat anti-Rat IgG1, IgG2a, IgG2b, IgG2c (Fc Region) Antibody (HRP)

Catalog No. ABIN2623505

Product Details

Target: IgG1, IgG2a, IgG2b, IgG2c

Binding Specificity: Fc Region

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunocytochemistry (ICC), Dot Blot (DB), Immunodiffusion (ID), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Specificity: Rat IgG, Fc specific.

Purification: Purified IgG

Immunogen: Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA, ICC, ID, IHC, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and gG/Fab fragments or any other serum protein. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin known to be of the IgG isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgG in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgG1, IgG2a, IgG2b, IgG2c

Alternative Name: IgG1 + IgG2a + IgG2b + IgG2c

Background: This antibody detects: IgG1 + IgG2a + IgG2b + IgG2c Fc