CD32/CD16 antibody (Biotin)

Catalog No. ABIN263930

Product Details anti-CD32/CD16 Antibody (Biotin)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Characteristics: Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, Fc-gamma RIII, FcRIII,CD16a, FcR mouse Seroblock, FCGR3A, FCG3, IGFR3, Fc gamma receptor IIB, Fc-gamma-RIIB,FcRII, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinityimmunoglobulin gamma Fc region receptor III-B

Clone: KT1632

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry (1 g is the maximum amount of reagent required per 10^6 cells). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10^6 cells per testsAntibody Concentration Used: 0.1 g/10^6 cellsIsotypic Control: FITC Rat IgG2a.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C for up to one month ore (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32 (CD32/CD16 Products)

Background: The lymphocyte Fc-gamma Receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc-gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc-gammaR+ cells, monoclonal antibodies to the Fc-gammaII/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc-gamma R for parasitic invasion.Synonyms: CD16a, FCG3, FCGR3A, Fc gamma receptor IIB, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, Fc-gamma-RIIB, FcR mouse Seroblock, FcRII, FcRIII, FcRIIIa, Fcgr2, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-B, Ly-17