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OCT4 antibody (POU Class 5 Homeobox 1)

Details for Product anti-POU5F1 Antibody No. ABIN263988, Supplier: Log in to see
Antigen
  • POU5F1L
  • POU5F1
  • OCT3
  • OCT4
  • OTF-3
  • OTF3
  • OTF4
  • Oct-3
  • Oct-4
  • NF-A3
  • Oct-3/4
  • Oct3
  • Oct3/4
  • Oct4
  • Otf-3
  • Otf-4
  • Otf3
  • Otf3-rs7
  • Otf3g
  • Otf4
  • gp9
  • gp-9
  • oct4
  • pou2
  • POU-2
  • cb197
  • spg/pou2
  • chunp6868
  • wu:fd18d06
Reactivity
Human
304
107
45
10
10
10
8
5
5
5
3
3
3
2
1
1
Host
Mouse
215
91
30
11
Clonality (Clone)
Monoclonal ()
Conjugate
This OCT4 antibody is un-conjugated
12
11
10
10
6
6
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
263
157
54
49
34
33
28
19
12
12
8
4
3
3
3
2
2
1
Supplier
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Immunogen BALB/C mice injected with synthetic peptide of human OCT-4 origin.
Clone NRG1-1
Isotype IgG2b
Specificity OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-bindingtranscription factor 3, Oct-3, Oct-4
Purification Purified
Alternative Name OCT3/4 (POU5F1 Antibody Abstract)
Background OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
Gene ID 5460
UniProt Q01860
Application Notes Suitable for Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/100 in an ABC method. Positive Control: Seminoma. Cellular Localization: Nuclear.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Staining ProtocolWe suggest an incubation period of 30 minutes at room temperature. However, dependingupon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require
Restrictions For Research Use only
Format Liquid
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-OCT4 antibody (POU Class 5 Homeobox 1) (ABIN263988) Formalin fixed paraffin embedded Testicular Seminoma stained with OCT4 antibody Cat.-...
Background publications Jones, MacLennan, Bonnin et al.: "Screening for intratubular germ cell neoplasia of the testis using OCT4 immunohistochemistry." in: The American journal of surgical pathology, Vol. 30, Issue 11, pp. 1427-31, 2006 (PubMed).

Lau, Chang: "OCT4: a sensitive and specific immunohistochemical marker for metastatic germ cell tumors." in: Advances in anatomic pathology, Vol. 13, Issue 2, pp. 76-9, 2006 (PubMed).

Hattab, Tu, Wilson et al.: "OCT4 immunohistochemistry is superior to placental alkaline phosphatase (PLAP) in the diagnosis of central nervous system germinoma." in: The American journal of surgical pathology, Vol. 29, Issue 3, pp. 368-71, 2005 (PubMed).