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ITGAL antibody (Integrin alpha-L) (Biotin)

Details for Product anti-ITGAL Antibody No. ABIN264308, Supplier: Log in to see
Antigen
  • (p180)
  • Cd11a
  • LFA-1
  • LFA-1A
  • Ly-15
  • Ly-21
  • CD11A
  • LFA1A
  • CD11a
Reactivity
Human
315
201
36
15
11
11
10
8
4
3
Host
Mouse
335
173
58
16
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is conjugated to Biotin
94
79
46
16
13
6
6
5
4
3
3
3
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
461
154
99
98
67
65
47
27
16
12
8
6
6
4
3
2
2
2
1
1
1
1
Supplier
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Clone OFYS
Isotype IgG2a
Specificity This antibody reacts with LFA-1 alpha. This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
Characteristics Synonyms: Integrin alpha-L, LFA1, LFA-1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocytefunction-associated molecule 1 alpha chain, CD11 antigen-like family member A
Alternative Name CD11a / ITGAL (ITGAL Antibody Abstract)
Background CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
Gene ID 3683
UniProt P20701
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry. Characterization of LFA-1 immunodeficiency. Monitoring of subsets in peripheral blood. Graft-versus-host disease. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
Restrictions For Research Use only
Buffer PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Anderson, Springer: "Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins." in: Annual review of medicine, Vol. 38, pp. 175-94, 1987 (PubMed).

van Dijken, Ghayur, Mauch et al.: "Evidence that anti-LFA-1 in vivo improves engraftment and survival after allogeneic bone marrow transplantation." in: Transplantation, Vol. 49, Issue 5, pp. 882-6, 1990 (PubMed).

Patarroyo, Prieto, Rincon et al.: "Leukocyte-cell adhesion: a molecular process fundamental in leukocyte physiology." in: Immunological reviews, Vol. 114, pp. 67-108, 1990 (PubMed).

Arnaout: "Leukocyte adhesion molecules deficiency: its structural basis, pathophysiology and implications for modulating the inflammatory response." in: Immunological reviews, Vol. 114, pp. 145-80, 1990 (PubMed).

Hartnell, Moqbel, Walsh et al.: "Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils." in: Immunology, Vol. 69, Issue 2, pp. 264-70, 1990 (PubMed).

Heiskala, Patarroyo, Timonen: "Participation of CD11a-c/CD18 and RGD-recognizing adhesion molecules in the binding of LGL to fibroblasts. Evidence for the role of CD11a in the fibroblast-mediated inactivation of NK cells." in: Scandinavian journal of immunology, Vol. 32, Issue 2, pp. 137-47, 1990 (PubMed).

Pedrinaci, Ruiz-Cabello, Gomez et al.: "Protein kinase C-mediated regulation of the expression of CD14 and CD11/CD18 in U937 cells." in: International journal of cancer. Journal international du cancer, Vol. 45, Issue 2, pp. 294-8, 1990 (PubMed).