Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.
NAPA
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Western Blot: 0.5-5 μg/mL. Immunoprecipitationon native and denatured samples: 2-10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol without preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.Synonyms: Alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, NAPA, SNAP-alpha, SNAPA