TP53I3 antibody

Catalog No. ABIN264400

This Mouse Monoclonal antibody specifically detects TP53I3 in WB. It exhibits reactivity toward Human.

Quick Overview for TP53I3 antibody (ABIN264400)

Target: TP53I3 (Tumor Protein P53 Inducible Protein 3 (TP53I3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TP53I3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 10A2

Product Details anti-TP53I3 Antibody

Specificity: Recognizes PIG3 (p53 inducible gene 3).

Characteristics: Synonyms: p53-induced gene 3 protein, PIG3, Tumor protein p53-inducible protein 3, Quinoneoxidoreductase PIG3

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman PIG-3 protein. Genename: TP53I3

Isotype: IgG1

Application Details

Application Notes: Western Blot (5-10 μg/mL). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for TP53I3

Target: TP53I3 (Tumor Protein P53 Inducible Protein 3 (TP53I3))

Alternative Name: TP53I3

Background: P53 Inducible Gene-3 (PIG-3) may be a long-lived reporter, which may be useful for detecting transient activation of p53. The p53 tumor suppressor is the most commonly mutated gene in human cancers. The p53 protein, which is stabilized in response to different biological checkpoints, is activated by DNA damage, hypoxia, viral infection, or oncogene activation resulting in efects such as cell cycle arrest, apoptosis, senescence, differentiation, and antiangiogenesis. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others. The p53-inducible gene 3 (PIG3), was recently identified in a screen for genes induced by p53 before the onset of apoptosis. PIG3 shares significant homology with the oxidoreductases from several species. PIG3 protein is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents. The induction of PIG3 by p53 occurrs with delayed kinetics as compared with other p53 downstream targets, such as p21 and MDM2. PIG3 levels are increased during p53-mediated growth arrest. Elevated levels of PIG3 are maintained in cells that resume cycling in the absence of ectopic p53 expression.Synonyms: PIG3, Quinone oxidoreductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein

Gene ID: 9540

UniProt: Q53FA7

Pathways: p53 Signaling