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SNAP25 antibody (Synaptosomal-Associated Protein, 25kDa)

Details for Product anti-SNAP25 Antibody No. ABIN264461, Supplier: Log in to see
Antigen
  • bA416N4.2
  • Bdr
  • dJ1068F16.2
  • fj55h11
  • ric-4
  • RIC-4
  • ric4
  • RIC4
  • SEC9
  • sec9
  • SNAP
  • SNAP-25
  • snap-25
  • SNAP-25a
  • SNAP-25B
  • snap25
  • sp
  • wu:fj55h11
Reactivity
Chicken, Cow (Bovine), Human, Monkey, Mouse (Murine), Rat (Rattus)
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108
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15
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10
7
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3
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2
2
1
1
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Host
Mouse
139
127
23
7
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SNAP25 antibody is un-conjugated
10
9
7
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7
2
2
2
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2
2
2
2
2
2
2
1
1
1
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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149
93
64
45
44
27
18
15
11
11
8
2
2
2
1
Supplier
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Supplier Product No.
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Immunogen Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withpurified rat SNAP25 and mouse NS1 cells.
Clone B-29-F9
Isotype IgG1
Specificity Does not stain reduced proteins.
Characteristics Synonyms: SNAP-25, SUP, SNAP, Super Protein, Synaptosomal-associated protein 25,Synaptosomal-associated 25 kDa protein
Purification Purified
Alternative Name SNAP25 (SNAP25 Antibody Abstract)
Background SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.Synonyms: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4
Gene ID 6616
UniProt P60880
Research Area Cancer, Neurology
Application Notes Western blot (1-5 μg/mL). Immunohistochemistry on Paraformaldehyde fixed CNS tissues (1-5 μg/mL). Positive Control: Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Gerona, Larsen, Kowalchyk, Martin: "The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6328-36, 2000

McMahon, Südhof: "Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2213-7, 1995

Zhao, Hashida, Takahashi, Sakaki: "Cloning and sequence analysis of the human SNAP25 cDNA." in: Gene, Vol. 145, Issue 2, pp. 313-4, 1994

Ritchie, Thomas, Coulter: "A nerve terminal protein with a selective distribution in spinal cord and brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 8, pp. 2697-709, 1989