SOD1 antibody

Catalog No. ABIN264856

This Rabbit Polyclonal antibody specifically detects SOD1 in WB, IP, IF, IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Sheep, Pig, Monkey, Dog, Hamster, Coral and Xenopus laevis.

Quick Overview for SOD1 antibody (ABIN264856)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Pig, Monkey, Dog, Hamster, Coral, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-SOD1 Antibody

Specificity: Detects 23 kDa (Human) and 19 kDa (Other species) proteins, corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS-PAGE immunoblots.

Purification: Antigen Affinity Chromatography

Immunogen: Human Cu/Zn SOD

Isotype: IgG

Application Details

Application Notes: Western blot (6,8): 0.2 μg/mL was sufficient for detection of Cu/Zn SOD in 20 g of HeLacell lysate. Immunoprecipitation (8). EIA (6). Immunohistochemistry on frozen sections(7).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS ( pH 7.0), 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: Superoxide Dismutase 1 / SOD1

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2¯ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

Gene ID: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis