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Lysine (lys) (acetylated) antibody

Details for Product No. ABIN264880, Supplier: Log in to see
Antigen
Epitope
acetylated
34
8
4
4
4
2
1
1
Reactivity
Chemical
50
5
4
3
3
3
2
Host
Rabbit
31
22
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
46
36
32
29
23
19
12
5
2
1
Supplier
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Immunogen Acetylated KLH Conjugated
Isotype IgG
Purification Affinity Chromatography
Alternative Name Lysine
Target Type Chemical
Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N?-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).Synonyms: AcK, acetyl Lysine, acetyl-Lysine
Research Area Metabolism
Application Notes Western blot (6). Immunoprecipitation (7). ELISA. Immonoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Lysine (lys) (acetylated) antibody (ABIN264880) anti-Lysine (lys) (acetylated) antibody
 image for anti-Lysine (lys) (acetylated) antibody (ABIN264880) anti-Lysine (lys) (acetylated) antibody (Image 2)
Background publications Ishiguro, Ando, Maeda, Watanabe, Goto: "Cutting edge: tubulin ? functions as an adaptor in NFAT-importin ? interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 5, pp. 2710-3, 2011 (PubMed).

Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).

Yang: "Lysine acetylation and the bromodomain: a new partnership for signaling." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 10, pp. 1076-87, 2004 (PubMed).

Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Chan, Krstic-Demonacos, Smith, Demonacos, La Thangue: "Acetylation control of the retinoblastoma tumour-suppressor protein." in: Nature cell biology, Vol. 3, Issue 7, pp. 667-74, 2001 (PubMed).

Martínez-Balbás, Bauer, Nielsen, Brehm, Kouzarides: "Regulation of E2F1 activity by acetylation." in: The EMBO journal, Vol. 19, Issue 4, pp. 662-71, 2000 (PubMed).

Hassig, Schreiber: "Nuclear histone acetylases and deacetylases and transcriptional regulation: HATs off to HDACs." in: Current opinion in chemical biology, Vol. 1, Issue 3, pp. 300-8, 1998 (PubMed).