S1PR1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 5-10 μg/mL, Detects an approximately 44 kDa band in RH7777 cellstransfected with full length human EDG-1. Due to low expression of EDG receptors, werecommend use of Pierce Femto Signal substrate for western blot development. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
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Cummings, Parinandi, Zaiman, Wang, Usatyuk, Garcia, Natarajan: "Phospholipase D activation by sphingosine 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 30227-35, (2002) (PubMed).
Graeler, Goetzl: "Activation-regulated expression and chemotactic function of sphingosine 1-phosphate receptors in mouse splenic T cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 14, pp. 1874-8, (2002) (PubMed).
EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocytecontaining and other tissues.Synonyms: CHEDG1, D1S3362, ECGF1, EDG1, Endothelial differentiation G-protein coupled receptor 1, FLJ58121, S1P receptor 1, S1PR1, Sphingosine 1-phosphate receptor 1