S1PR1 antibody (C-Term)

Catalog No. ABIN264982

Product Details anti-S1PR1 Antibody

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to EDG1.

Purification: Purified

Immunogen: Synthetic peptide derived from the C-terminal of the EDG-1 receptor

Isotype: IgG

Application Details

Application Notes: Western Blot: 5-10 μg/mL, Detects an approximately 44 kDa band in RH7777 cellstransfected with full length human EDG-1. Due to low expression of EDG receptors, werecommend use of Pierce Femto Signal substrate for western blot development.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

References for anti-S1PR1 antibody (ABIN264982)

Zhang, Loyd, Randall, Waddell, Kriwacki, Ney: "A short linear motif in BNIP3L (NIX) mediates mitochondrial clearance in reticulocytes." in: Autophagy, Vol. 8, Issue 9, pp. 1325-32, (2013) (PubMed).

Goparaju, Jolly, Watterson, Bektas, Alvarez, Sarkar, Mel, Ishii, Chun, Milstien, Spiegel: "The S1P2 receptor negatively regulates platelet-derived growth factor-induced motility and proliferation." in: Molecular and cellular biology, Vol. 25, Issue 10, pp. 4237-49, (2005) (PubMed).

Matsushita, Morrell, Lowenstein: "Sphingosine 1-phosphate activates Weibel-Palade body exocytosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 31, pp. 11483-7, (2004) (PubMed).

Toman, Payne, Watterson, Maceyka, Lee, Milstien, Bigbee, Spiegel: "Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extension." in: The Journal of cell biology, Vol. 166, Issue 3, pp. 381-92, (2004) (PubMed).

Yamaguchi, Kitayama, Takuwa, Arikawa, Inoki, Takehara, Nagawa, Takuwa: "Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and haematogenous metastasis of melanoma cells." in: The Biochemical journal, Vol. 374, Issue Pt 3, pp. 715-22, (2003) (PubMed).

Cummings, Parinandi, Zaiman, Wang, Usatyuk, Garcia, Natarajan: "Phospholipase D activation by sphingosine 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 30227-35, (2002) (PubMed).

Graeler, Goetzl: "Activation-regulated expression and chemotactic function of sphingosine 1-phosphate receptors in mouse splenic T cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 14, pp. 1874-8, (2002) (PubMed).

Target Details for S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Alternative Name: EDG-1 / S1PR1 (S1PR1 Products)

Synonyms: CD363 antibody, CHEDG1 antibody, D1S3362 antibody, ECGF1 antibody, EDG-1 antibody, EDG1 antibody, S1P1 antibody, AI849002 antibody, Edg1 antibody, Lpb1 antibody, S1p antibody, S1p1 antibody, S1P receptor 1 antibody, S1P receptor Edg-1 antibody, edg1 antibody, etID43307.22 antibody, s1p1 antibody, wu:fb98f05 antibody, s1pr1 antibody, xts1p1 antibody, sphingosine-1-phosphate receptor 1 antibody, sphingosine-1-phosphate receptor 1 L homeolog antibody, S1PR1 antibody, S1pr1 antibody, s1pr1 antibody, s1pr1.L antibody

Background: EDG-1 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-1 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-1 is expressed in cardiovascular, leukocytecontaining and other tissues.Synonyms: CHEDG1, D1S3362, ECGF1, EDG1, Endothelial differentiation G-protein coupled receptor 1, FLJ58121, S1P receptor 1, S1PR1, Sphingosine 1-phosphate receptor 1

Gene ID: 1901

NCBI Accession: NP_001391

UniProt: P21453

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2