JUP antibody

Catalog No. ABIN265337

The Rabbit Polyclonal anti-JUP antibody is suitable to detect JUP in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for JUP antibody (ABIN265337)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JUP Antibody

Specificity: This antibody detects endogenous levels of Catenin-γ / Junction plakoglobin protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Junction Plakoglobin

Background: The catenins, α, β and γ, are proteins which bind to the highly conserved,intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherincomplexes play an important role mediating cellular adhesion. α-catenin was initially described as an E-cadherin associated protein, and since has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. β-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. β-catenin has also been found in complexes with the tumor suppressor protein APC. γ-catenin, also known as plakoglobin, binds with α-catenin and N-cadherin. It has been shown that the transmembrane phosphatase PTPμ associates with catenin/cadherin complexes and may regulate complex signaling.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP

Molecular Weight: approx. 82 kDa

Gene ID: 3728

NCBI Accession: NP_002221

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location