FANCD2 antibody

Catalog No. ABIN265413

The Rabbit Polyclonal anti-FANCD2 antibody (ABIN265413) specifically detects FANCD2 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FANCD2 antibody (ABIN265413)

Target: FANCD2 (Fanconi Anemia, Complementation Group D2 (FANCD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FANCD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FANCD2 Antibody

Specificity: This antibody detects endogenous levels of FANCD2 protein (region surrounding Pro216).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FANCD2

Target: FANCD2 (Fanconi Anemia, Complementation Group D2 (FANCD2))

Alternative Name: FANCD2

Background: The Fanconi anemia (FA) proteins lack sequence homologies or motifs that could point to a molecular function. Phosphorylation of FANC(Fanconi anemia complementation group) proteins are thought to be important for the function of the FA pathway. Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci. In male meiosis, FANCD2 also co-localizes with BRCA1 at synaptonemal complexes. The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1,451 amino acid nuclear protein that exists as two protein isoforms.Synonyms: FACD, FACD2, Fanconi anemia group D2 protein

Molecular Weight: approx. 166 kDa

Gene ID: 2177

NCBI Accession: NP_001018125

UniProt: Q9BXW9

Pathways: DNA Damage Repair