HSPA9 antibody

Catalog No. ABIN265446

Product Details anti-HSPA9 Antibody

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of GRP75/HSPA9 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000approx. 1/40000. Western Bot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200. Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HSPA9

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Alternative Name: Grp75 / HSPA9 (HSPA9 Products)

Synonyms: APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CSA antibody, GRP-75 antibody, GRP75 antibody, HSPA9B antibody, MOT antibody, MOT2 antibody, MTHSP75 antibody, PBP74 antibody, Hspa9a antibody, csa antibody, grp-75 antibody, grp75 antibody, hspa9 antibody, hspa9b antibody, mortalin antibody, mot antibody, mot2 antibody, pbp74 antibody, mot-2 antibody, mthsp75 antibody, 74kDa antibody, Csa antibody, Grp75 antibody, Hsc74 antibody, Hsp74 antibody, Hsp74a antibody, Mortalin antibody, Mot-2 antibody, Mot2 antibody, Mthsp70 antibody, Pbp74 antibody, cb740 antibody, crs antibody, wu:fc14d08 antibody, wu:fc27c10 antibody, wu:fc38a06 antibody, heat shock protein family A (Hsp70) member 4 antibody, heat shock protein family A (Hsp70) member 9 antibody, heat shock protein family A member 9 antibody, heat shock protein family A (Hsp70) member 9 S homeolog antibody, stress-70 protein, mitochondrial antibody, heat shock protein 9 antibody, heat shock protein Hsp9 antibody, HSPA4 antibody, HSPA9 antibody, Hspa9 antibody, hspa9.S antibody, hspa9 antibody, LOC577721 antibody, hsp9 antibody

Background: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.Synonyms: 75 kDa glucose-regulated protein, HSPA9B, Heat shock 70 kDa protein 9, MOT, Mortalin, PBP74, Peptide-binding protein 74, Stress-70 protein

Molecular Weight: approx. 90 kDa

Gene ID: 3313

NCBI Accession: NP_004125

UniProt: P38646