hCG antibody

Catalog No. ABIN265452

Product Details anti-hCG Antibody

Target: hCG (Chorionic Gonadotropin (hCG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This hCG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of hCG β protein. (region surrounding Asp131)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for hCG

Target: hCG (Chorionic Gonadotropin (hCG))

Abstract: hCG Products

Synonyms: CGB antibody, AW108023 antibody, HCG antibody, chorionic gonadotropin, beta polypeptide antibody, ubiquitin-like 3 antibody, chorionic gonadotropin beta subunit 5 antibody, CGB antibody, Ubl3 antibody, CGB5 antibody

Background: Choriogonadotropin is a hormone produced by the placenta in the first trimester of pregnancy and exists as a heterodimer formed from a unique β chain and an α chain common to all gonadotropins. The unique β chain confers biological specificity to choriogonadotropin, luteinizing hormone and follicle stimulating hormone. The secreted α subunit maps to human chromosome 6 and the β subunit of choriogonadotropin maps to human chromosome 19. Choriogonadotropin stimulates the ovaries to produce and maintain normal levels of the steroids essential for maintaining pregnancy, including estrogen and progesterone. Choriogonadotropin is a member of the cystine knot growth-factor superfamily, a group of proteins that contain a distinct arrangement of six cysteine residues and are expressed in placenta. The proper secretion and dimerization of choriogonadotropin depends on the conformation of the cystine knot, although biological activity is independent of this conformation.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta

Molecular Weight: approx. 18 kDa

Gene ID: 1082

NCBI Accession: NP_000728

UniProt: P01233