Nanog antibody

Catalog No. ABIN2666251

Product Details anti-Nanog Antibody

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Biochemical Assay (BCA)

Purification: The antibody was purified by affinity chromatography.

Clone: 16H3A48

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store undiluted at 4°C

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog (NANOG Products)

Synonyms: 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

Background: NANOG is a homeodomain-containing transcription factor that is essential for early embryonic development. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer cell lines, maintaining undifferentiated statuses of the cells and is downregulated upon differentiation. Si-RNA mediated knockdown of NANOG in ES cells induces lineage differentiation. Overexpression of NANOG allows for the leukemia inhibitory factor (LIF)-independent selfrenewal in undifferentiated ES cells. Nanog functionally works with OCT4 and SOX2 to support pluripotency and selfrenewal in ES cells and the expression of NANOG is regulated by OCT4/SOX2 heterodimers. Expression of NANOG has been detected in several types of tumor cells. The expression level of NANOG in cancer cells has been shown to be correlated with the malignancy of cancers.

Pathways: Stem Cell Maintenance