IL4 Receptor antibody

Catalog No. ABIN2688941

Product Details anti-IL4 Receptor Antibody

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by normal splenocyres from BALB/c and C57BL/6 mice. Spleen cells from BALB/c (left panel) and C57BL/6 (right panel) mice were treated with ACK lysis buffer, were washed, and were labeled with purified Fc Block (Cat. No. 553142, Rat IgG2b anti-mouse CD16/CD32) to block mouse Fc receptors. The cells were then stained with mIL4R-M1 (1 μg) followed by biotinylated RG7/1.30 (mouse anti-rat IgG2α, 0.25 μg, Cat. No. 553894) and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the mIL4R-M1 antibody (filled histograms) is compared to staining with the secondary reagents alone (open histograms).

BD Pharmingen™ Purified Rat Anti-Mouse CD124 - Purified - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: MIL4R

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL4 Receptor antibody (ABIN2688941)

Gessner, Röllinghoff: "Biologic functions and signaling of the interleukin-4 receptor complexes." in: Immunobiology, Vol. 201, Issue 3-4, pp. 285-307, (2000) (PubMed).

Kubo, Yamashita, Abe, Tada, Okumura, Ransom, Nakayama: "CD28 costimulation accelerates IL-4 receptor sensitivity and IL-4-mediated Th2 differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2432-42, (1999) (PubMed).

Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, (1997) (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, (1994) (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, (1991) (PubMed).

Beckmann, Schooley, Gallis, Vanden Bos, Friend, Alpert, Raunio, Prickett, Baker, Park: "Monoclonal antibodies block murine IL-4 receptor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 11, pp. 4212-7, (1990) (PubMed).

Mosley, Beckmann, March, Idzerda, Gimpel, VandenBos, Friend, Alpert, Anderson, Jackson: "The murine interleukin-4 receptor: molecular cloning and characterization of secreted and membrane bound forms." in: Cell, Vol. 59, Issue 2, pp. 335-48, (1989) (PubMed).

Lowenthal, Castle, Christiansen, Schreurs, Rennick, Arai, Hoy, Takebe, Howard: "Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 2, pp. 456-64, (1988) (PubMed).

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: CD124 (IL4R Products)

Synonyms: IL4R antibody, il-4ra antibody, zgc:113063 antibody, CD124 antibody, IL-4RA antibody, IL4RA antibody, Il4ra antibody, IL4 antibody, Il4r antibody, interleukin 4 receptor antibody, interleukin 4 receptor, tandem duplicate 1 antibody, interleukin 4 receptor, alpha antibody, IL4R antibody, il4r.1 antibody, Il4r antibody, Il4ra antibody

Background: Synonyms: IL-4 Receptor α chain

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process