F11R antibody

Catalog No. ABIN2689190

Product Details anti-F11R Antibody

Target: F11R (F11 Receptor (F11R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F11R antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The M.Ab.F11 monoclonal antibody specifically binds to CD321 which is also known as JAM-1 (Junctional adhesion molecule 1), Junctional adhesion molecule A (JAM-A), and F11 Receptor (F11R). CD321 is a 32-35 kDa type I transmembrane glycoprotein that includes two extracellular immunoglobulin-like domains. CD321 is expressed on platelets, leucocytes, red blood cells, endothelial cells, epithelial cells, and various cell lines. CD321 functions as an adhesion receptor molecule on platelets. It also supports the tight junction formation between endothelial cells, where it may regulate the transendothelial migration of leucocytes, and epithelial cells. M.Ab.F11 is a stimulatory antibody that can induce morphological changes, granule secretion, and aggregation in human platelets. Flow cytometric analysis of human CD321 staining on platelets. Peripheral blood platelets were stained with either Purified Mouse Anti-Human CD321 (JAM-1) (Cat. No. 552147, solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746, dashed line histogram), followed by second step staining with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988).

BD Pharmingen™ Purified Mouse Anti-Human CD321 (JAM-1) - Purified - Clone M.Ab.F11 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: M-Ab-F11

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-F11R antibody (ABIN2689190)

Florian, Sonneck, Czerny, Hennersdorf, Hauswirth, Bühring, Valent: "Detection of novel leukocyte differentiation antigens on basophils and mast cells by HLDA8 antibodies." in: Allergy, Vol. 61, Issue 9, pp. 1054-62, (2006) (PubMed).

Horváth, Drbel, Angelisová, Hilgert, Horejsí: "Non-lineage antigens: section report." in: Cellular immunology, Vol. 236, Issue 1-2, pp. 42-7, (2005) (PubMed).

Babinska, Kedees, Athar, Sobocki, Sobocka, Ahmed, Ehrlich, Hussain, Kornecki: "Two regions of the human platelet F11-receptor (F11R) are critical for platelet aggregation, potentiation and adhesion." in: Thrombosis and haemostasis, Vol. 87, Issue 4, pp. 712-21, (2002) (PubMed).

Sobocka, Sobocki, Banerjee, Weiss, Rushbrook, Norin, Hartwig, Salifu, Markell, Babinska, Ehrlich, Kornecki: "Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation." in: Blood, Vol. 95, Issue 8, pp. 2600-9, (2000) (PubMed).

Kornecki, Walkowiak, Naik, Ehrlich: "Activation of human platelets by a stimulatory monoclonal antibody." in: The Journal of biological chemistry, Vol. 265, Issue 17, pp. 10042-8, (1990) (PubMed).

Target Details for F11R

Target: F11R (F11 Receptor (F11R))

Alternative Name: CD321 JAM-1 (F11R Products)

Synonyms: CD321 antibody, JAM antibody, JAM1 antibody, JAMA antibody, JCAM antibody, KAT antibody, PAM-1 antibody, 9130004G24 antibody, AA638916 antibody, ESTM33 antibody, JAM-1 antibody, JAM-A antibody, Jcam antibody, Jcam1 antibody, Ly106 antibody, Jam1 antibody, F11 receptor antibody, F11R antibody, F11r antibody

Background: Synonyms: JAM-1, Platelet F11 receptor, F11R, JAM-A, PAM-1

Pathways: Cell-Cell Junction Organization