CD34 antibody (Biotin)

Catalog No. ABIN2689204

Product Details anti-CD34 Antibody (Biotin)

Target: CD34

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3 % of cells are stained with 2E9 mAb, whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

BD Pharmingen™ Biotin Mouse Anti-Canine CD34 - Biotin - Clone 2E9 - Isotype Mouse IgG1, κ - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CD34-mouse IgG2a fusion protein and canine myelomonocytic leukemia ML3

Clone: 2E9

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD34 antibody (Biotin) (ABIN2689204)

Georges, Storb, Bruno, Brodie, Thompson, Taranova, Zaucha, Little, Zellmer, Moore, Gooley, Sale, Kiem, Sandmaier, Lyons, Nash: "Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes." in: Blood, Vol. 98, Issue 12, pp. 3447-55, (2001) (PubMed).

Goerner, Horn, Peterson, Kurre, Storb, Rasko, Kiem: "Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors." in: Blood, Vol. 98, Issue 7, pp. 2065-70, (2001) (PubMed).

Niemeyer, Hudson, Bridgman, Spano, Nash, Lothrop: "Isolation and characterization of canine hematopoietic progenitor cells." in: Experimental hematology, Vol. 29, Issue 6, pp. 686-93, (2001) (PubMed).

Bhattacharya, McSweeney, Shi, Bruno, Ishida, Nash, Storb, Sauvage, Hammond, Wu: "Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells." in: Blood, Vol. 95, Issue 2, pp. 581-5, (2000) (PubMed).

Hägglund, McSweeney, Mathioudakis, Bruno, Georges, Gass, Moore, Sale, Storb, Nash: "Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells." in: Transplantation, Vol. 70, Issue 10, pp. 1437-42, (2000) (PubMed).

Bruno, Nash, Wallace, Gass, Thompson, Storb, McSweeney: "CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation." in: Transplantation, Vol. 68, Issue 3, pp. 338-44, (1999) (PubMed).

McSweeney, Rouleau, Wallace, Bruno, Andrews, Krizanac-Bengez, Sandmaier, Storb, Wayner, Nash: "Characterization of monoclonal antibodies that recognize canine CD34." in: Blood, Vol. 91, Issue 6, pp. 1977-86, (1998) (PubMed).

McSweeney, Rouleau, Storb, Bolles, Wallace, Beauchamp, Krizanac-Bengez, Moore, Sale, Sandmaier, de Revel, Appelbaum, Nash: "Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein." in: Blood, Vol. 88, Issue 6, pp. 1992-2003, (1996) (PubMed).

Baumheter, Singer, Henzel, Hemmerich, Renz, Rosen, Lasky: "Binding of L-selectin to the vascular sialomucin CD34." in: Science (New York, N.Y.), Vol. 262, Issue 5132, pp. 436-8, (1993) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: Synonyms: gp 105-120