CD79b antibody

Catalog No. ABIN2689398

Product Details anti-CD79b Antibody

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD79b antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The HM79b antibody reacts with an extracellular epitope of Ig β chain (Igβ or CD79b), a 35-40- kDa transmembrane protein which forms an 80-90- kDa disulfide-linked heterodimer with Ig α chain (Igα or CD79a, 30-35 kDa). On mature B lymphocytes, the CD79a/CD79b heterodimers are non-covalently associated with surface IgM to form the B-cell receptor complex (BCR). The presence of CD79a/CD79b is necessary for surface expression of the BCR and signal transduction via the BCR in B lymphocytes and pre-B cells. It was recently reported that CD79b may be expressed on the cell surface preceding the appearance of surface IgM during B-lymphocyte development. At this pro-B-cell stage, CD79b participates in signal transduction involved in the regulation of B-cell development. It should be noted that multi-parameter flow cytometric analyses of bone marrow suspensions performed have been unable to detect surface staining by HM79b mAb on CD45R/B220+ IgM- cells. Two-color analysis of the expression of CD79b on mouse bone marrow cells. A single-cell suspension of BALB/c bone marrow was simultaneously stained with FITC-conjugated anti-mouse CD45R/B220 RA3-6B2 (Cat. No. 553087/553088) and purified HM79b monoclonal antibodies, followed by PE-conjugated anti-hamster IgG cocktail (Cat. No. 554056). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD79b - Purified - Clone HM79b (also known as HM79-16) - Isotype Armenian Hamster IgG2, λ1 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse B lymphoma, WEHI-123

Clone: HM79b

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD79b antibody (ABIN2689398)

Koyama, Ishihara, Karasuyama, Cordell, Iwamoto, Nakamura: "CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain." in: International immunology, Vol. 9, Issue 11, pp. 1767-72, (1998) (PubMed).

Nagata, Nakamura, Kitamura, Kuramochi, Taki, Campbell, Karasuyama: "The Ig alpha/Igbeta heterodimer on mu-negative proB cells is competent for transducing signals to induce early B cell differentiation." in: Immunity, Vol. 7, Issue 4, pp. 559-70, (1997) (PubMed).

Gong, Nussenzweig: "Regulation of an early developmental checkpoint in the B cell pathway by Ig beta." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 411-4, (1996) (PubMed).

Papavasiliou, Jankovic, Suh, Nussenzweig: "The cytoplasmic domains of immunoglobulin (Ig) alpha and Ig beta can independently induce the precursor B cell transition and allelic exclusion." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1389-94, (1995) (PubMed).

Papavasiliou, Misulovin, Suh, Nussenzweig: "The role of Ig beta in precursor B cell transition and allelic exclusion." in: Science (New York, N.Y.), Vol. 268, Issue 5209, pp. 408-11, (1995) (PubMed).

Pleiman, DAmbrosio, Cambier: "The B-cell antigen receptor complex: structure and signal transduction." in: Immunology today, Vol. 15, Issue 9, pp. 393-9, (1994) (PubMed).

Reth: "Antigen receptors on B lymphocytes." in: Annual review of immunology, Vol. 10, pp. 97-121, (1992) (PubMed).

Target Details for CD79b

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Alternative Name: CD79b (CD79B Products)

Synonyms: B29 antibody, Ig-beta antibody, Igb antibody, Igbeta antibody, AGM6 antibody, IGB antibody, CD79B antigen antibody, CD79b molecule antibody, Cd79b antibody, CD79B antibody

Background: Synonyms: Igβ

Pathways: BCR Signaling