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IL-4 antibody

IL4 Reactivity: Mouse WB, ELISA, Neut Host: Rat Monoclonal BVD4 unconjugated
Catalog No. ABIN2689762
  • Target See all IL-4 (IL4) Antibodies
    IL-4 (IL4) (Interleukin 4 (IL4))
    Reactivity
    • 135
    • 57
    • 22
    • 14
    • 11
    • 10
    • 10
    • 8
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Mouse
    Host
    • 109
    • 59
    • 56
    • 7
    • 3
    • 2
    Rat
    Clonality
    • 126
    • 107
    • 1
    Monoclonal
    Conjugate
    • 138
    • 33
    • 16
    • 13
    • 10
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-4 antibody is un-conjugated
    Application
    • 118
    • 110
    • 70
    • 61
    • 25
    • 20
    • 19
    • 16
    • 13
    • 13
    • 12
    • 11
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Neutralization (Neut)
    Brand
    BD Pharmingen™
    Characteristics
    The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody. This antibody is routinely tested by intracellular staining and sandwich ELISA. Other applications were tested during antibody development only or reported in the literature. Expression of IL-4 by MiCK-2 positive control cells. MiCK-2 positive control cells (Cat. No. 554653) were stained with 0.125 μg of FITC-conjugated rat anti-mouse IL-4 antibody by using the BD Pharmingen staining protocol (see left panel). To demonstrate specificity of staining, the binding of FITC-BVD4-1D11 was blocked by the preincubation of the conjugated antibody with recombinant mouse IL-4 (0.25 μg, Cat. No. 550067, see middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled BVD4-1D11 antibody (5 μg, Cat. No. 554386, see right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.

    BD Pharmingen™ Purified Rat Anti-Mouse IL-4 - Purified - Clone BVD4-1D11 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Recombinant Mouse IL-4
    Clone
    BVD4
    Isotype
    IgG2b
    Top Product
    Discover our top product IL4 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

    Litton, Sander, Murphy, OGarra, Abrams: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).

    Finkelman, Madden, Morris, Holmes, Boiani, Katona, Maliszewski: "Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1235-44, (1993) (PubMed).

    Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

  • Target
    IL-4 (IL4) (Interleukin 4 (IL4))
    Alternative Name
    IL-4 (IL4 Products)
    Synonyms
    IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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