»
Antibody
»
anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 XRCC3
X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) antibody
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (23), Immunofluorescence (IF) (15), Flow Cytometry (FACS) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (7), ELISA (Detection) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (EM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
| Catalog no. |
ABIN271981 |
| Quantity |
100 µg (1,0 mg/ml) (Variants) |
| Price |
340.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Characteristics
|
Synonyms: DNA repair protein XRCC3, X-ray repair cross-complementing protein 3
|
|
Alternative name
|
XRCC3
|
|
Swiss-Prot
|
O43542
|
|
Description
|
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficientlyrepairing and maintaining genetic stability following DNA base damage. These genes sharesequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein thatfacilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNApolymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved inmaintaining chromosome stability during cell division. XRCC2 is required for efficientrepair of DNA double-strand breaks by homologous recombination between sisterchromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 duringrecombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentiallybinds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances itsjoining activity, and it is also involved in V(D)J recombination. Any defect in one of theknown components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80,DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement processand early block in both T and B cell maturation.
|
|
Specificity
|
XRCC3 (Q67) antibody detects endogenous levels of XRCC3 protein. Species: Human. Others not tested.
|
|
Application Notes
|
ELISA: 1: 5000~1: 10000. WB: 1: 500~1: 1000. IHC: 1: 50~1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
|
|
Concentration
|
1,0 mg/ml
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
|
|
Storage
|
Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
|
|
Research Area
|
Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3)", type "Antibodies"
|
Hosts
|
Rabbit (33), Mouse (4)
|
|
Reactivities
|
Human (35), Mouse (Murine) (15)
|
|
Applications
|
Western Blotting (WB) (23), Immunofluorescence (IF) (15), Flow Cytometry (FACS) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (7), ELISA (Detection) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (EM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
Center (2)
|
