X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4)

Synonyms: AW413319, AW545101, 2310057B22Rik, MGC95022, MGC73312, zgc:73312, MGC81443, XRCC4, MGC148661, DKFZp469B0634

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN271982

Additional Information

Characteristics: Synonyms: DNA repair protein XRCC4, X-ray repair cross-complementing protein 4

Alternative name: XRCC4

Swiss-Prot: Q13426

Cross-Reactivity: Human

Description: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficientlyrepairing and maintaining genetic stability following DNA base damage. These genes sharesequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a 70 kDa protein thatfacilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNApolymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved inmaintaining chromosome stability during cell division. XRCC2 is required for efficientrepair of DNA double-strand breaks by homologous recombination between sisterchromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 duringrecombinational repair. XRCC4 is an accessory factor of DNA ligase IV that preferentiallybinds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances itsjoining activity, and it is also involved in V(D)J recombination. Any defect in one of theknown components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80,DNA-PKCS, XRCC4 and DNA ligase IV) leads to abortion of the V(D)J rearrangement processand early block in both T and B cell maturation.

Specificity: XRCC4 (N291) antibody detects endogenous levels of XRCC4 protein. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1: 20000~1: 40000. Western blot: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only