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Antibodies
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anti-Polymerase (DNA Directed), alpha 1 (POLA1) antibody
Polymerase (DNA Directed), alpha 1 (POLA1) antibody
| Antigen |
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| Synonyms |
pola, cb678, pola1, POLA, POLA1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
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| Catalog no. |
ABIN272161 |
| Quantity |
100 µg (1,0 mg/ml) |
| Price |
340.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180
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Alternative name
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POLA1
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Gene ID
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5422
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Swiss-Prot
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P09884
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Cross-Reactivity
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Human
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Description
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DNA replication, recombination and repair, all of which are necessary for genomic stability,require the presence of exonucleases. In DNA replication, these enzymes are involved inthe processing of Okazaki fragments, whereas in DNA repair they function to excisedamaged DNA fragments and correct recombinational mismatches. These exonucleasesinclude the family of DNA polymerases. DNA pol α, β, δ and ε are involved in DNAreplication and repair. DNA pol δ and DNA pol ε are multisubunit enzymes, with DNA pol δconsisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA,and p50. The nuclear-encoded DNA pol γ is the only DNA polymerase required for thereplication of the mitochondrial DNA. DNA pol ζ is ubiquitously expressed in varioustissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol θis a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrandcrosslinks.
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Specificity
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DNA pol α (K111) antibody detects endogenous levels of DNA pol α protein. Species: Human, Mouse, Rat. Others not tested.
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Application Notes
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ELISA: 1: 20000~1: 40000. WB: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1,0 mg/ml
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Purification
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Aff - Purified
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
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Storage
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Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Polymerase (DNA Directed), alpha 1 (POLA1)", type "Antibodies"
