POLA1 antibody (N-Term)

Catalog No. ABIN7184445

Product Details anti-POLA1 Antibody

Target: POLA1 (DNA Polymerase alpha (POLA1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from N-terminal of Human DNA Polymerase alpha.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Alternative Name: POLA1 (POLA1 Products)

Synonyms: pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody

Background:

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Wong S.W., EMBO J. 7:37-47(1988).
Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991).
Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).

Aliases: DKFZp686K1672 antibody, DNA polymerase alpha 1 catalytic subunit antibody, DNA polymerase alpha catalytic subunit antibody, DNA polymerase alpha catalytic subunit p180 antibody, DNA polymerase alpha p180 subunit antibody, DPOLA_HUMAN antibody, OTTHUMP00000023089 antibody, OTTHUMP00000023090 antibody, P180 antibody, p180 subunit antibody, POLA antibody, POLA1 antibody, Polymerase (DNA directed), alpha 1, catalytic subunit antibody, Polymerase (DNA-directed), alpha (70kD) antibody

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome