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POLA1 antibody (N-Term)

POLA1 Reactivity: Human WB, ELISA, ICC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6258119
  • Target See all POLA1 Antibodies
    POLA1 (DNA Polymerase alpha (POLA1))
    Binding Specificity
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 31
    • 12
    • 11
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 38
    • 5
    • 1
    Rabbit
    Clonality
    • 40
    • 4
    Polyclonal
    Conjugate
    • 20
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    This POLA1 antibody is un-conjugated
    Application
    • 19
    • 11
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    DNA Polymerase alpha Antibody detects endogenous levels of total DNA Polymerase alpha.
    Predicted Reactivity
    Pig,Horse
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human DNA Polymerase alpha, corresponding to a region within N-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product POLA1 Primary Antibody
  • Application Notes
    WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    POLA1 (DNA Polymerase alpha (POLA1))
    Alternative Name
    POLA1 (POLA1 Products)
    Synonyms
    pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody
    Background

    Description: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).

    Gene: POLA1

    Molecular Weight
    165 kDa
    Gene ID
    5422
    UniProt
    P09884
    Pathways
    SARS-CoV-2 Protein Interactome
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