DCP1A antibody

Catalog No. ABIN272254

Product Details anti-DCP1A Antibody

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCP1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of SMIF protein. (region surrounding Ser133)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for DCP1A

Target: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Alternative Name: DCP1A (DCP1A Products)

Synonyms: DCP1A antibody, 1110066A22Rik antibody, 4930568L04Rik antibody, AU019772 antibody, D14Ertd817e antibody, Mitc1 antibody, SMIF antibody, RGD1561298 antibody, mitc1 antibody, HSA275986 antibody, Nbla00360 antibody, SMAD4IP1 antibody, smad4ip1 antibody, smif antibody, decapping mRNA 1A antibody, decapping mRNA 1A L homeolog antibody, DCP1A antibody, Dcp1a antibody, dcp1a antibody, dcp1a.L antibody

Background: Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.Synonyms: SMIF, Smad4-interacting transcriptional co-activator, Transcription factor SMIF, mRNA-decapping enzyme 1A

Molecular Weight: approx. 75 kDa

Gene ID: 55802

NCBI Accession: NP_060873

UniProt: Q9NPI6