FOXD3 antibody

Catalog No. ABIN272260

Product Details anti-FOXD3 Antibody

Target: FOXD3 (Forkhead Box D3 (FOXD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of FoxD3 protein. (region surrounding Arg233)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. Western blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.3, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXD3

Target: FOXD3 (Forkhead Box D3 (FOXD3))

Alternative Name: FOXD3 / HFH2 (FOXD3 Products)

Synonyms: AIS1 antibody, Genesis antibody, HFH2 antibody, VAMAS2 antibody, CWH3 antibody, Hfh2 antibody, fkd6 antibody, fkh6 antibody, forkhead-6 antibody, zgc:111934 antibody, XFD-6 antibody, xfoxd3 antibody, FOXD3 antibody, genesis antibody, hfh2 antibody, foxd3b antibody, foxd3b-A antibody, forkhead box D3 antibody, forkhead box D3 L homeolog antibody, forkhead box D3 S homeolog antibody, FOXD3 antibody, Foxd3 antibody, foxd3 antibody, foxd3.L antibody, foxd3.S antibody

Background: Embryonic stem cells require the forkhead transcriptional regulator FoxD3 for survival. Following gastrulation, FoxD3 generally gets downregulated, except in the neural crest. A variety of growth factors induce FoxD3 expression, including FGF8 and SNAIL, maintaining the effected cells in an undifferentiated state. Thus defects in FoxD3 induction may cause premature differentiation and/or migration-asociated birth defects.Synonyms: Forkhead box protein D3, HNF3/FH transcription factor genesis

Molecular Weight: approx. 48.0 kDa

Gene ID: 27022

NCBI Accession: NP_036315

UniProt: Q9UJU5