CD3 epsilon antibody (FITC)
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- Target See all CD3 epsilon (CD3E) Antibodies
- CD3 epsilon (CD3E)
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Reactivity
- Mouse
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Host
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Hamster
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Clonality
- Monoclonal
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Conjugate
- This CD3 epsilon antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Specificity
- Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
- Purification
- Protein G purified
- Immunogen
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Mouse BM10-37 T cell membrane prep.
Type of Immunogen: Membrane preparation - Clone
- 145-2C11
- Isotype
- IgG
- Top Product
- Discover our top product CD3E Primary Antibody
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- Application Notes
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Approved: ELISA, Flo, IP, WB
Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/FITC at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Synonyms
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- Background
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Name/Gene ID: CD3E
Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon - Gene ID
- 916
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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