The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
Cross-Reactivity (Details)
Human
Purification
Purified by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Endotoxin Level
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen
Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
TNFRSF10A
Reactivity: Human
FACS
Host: Mouse
Monoclonal
DR-4-02
APC
Application Notes
Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/mL in cultivation medium, final concentration of TRAIL: 20-200 ng/mL, it is recommended to add the antibody 15 min before addition of TRAIL. Flow cytometry: Recommended dilution: 2-4 μg/mL.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4
Preservative
Azide free
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).
Target
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
TNF receptor superfamily member 10a,TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).,DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1