SRF (N-Term) antibody

Details for Product No. ABIN2774664, Supplier: Log in to see
Antigen
  • AW049942
  • AW240594
  • MCM1
  • SRFX
  • XSRF
  • srf-A
  • RGD1559787
  • srf
  • srf1
  • serum response factor
  • serum response factor (c-fos serum response element-binding transcription factor)
  • serum response factor a
  • Srf
  • SRF
  • srf
  • srfa
Epitope
N-Term
23
20
9
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
154
112
78
5
4
4
3
2
2
1
1
1
1
1
Host
Rabbit
124
17
14
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
111
52
44
39
30
8
7
7
3
2
2
2
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SRF
Sequence ATGGYGPVSG AVSGAKPGKK TRGRVKIKME FIDNKLRRYT TFSKRKTGIM
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against SRF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name Serum response factor (SRF)
Background SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Alias Symbols: MCM1
Protein Interaction Partner: Camk2d, ZIC3, ELK1, SRFBP1, SRF, NFKB1, PRKDC, CIRBP, ALDH3A1, SUMO1, UBE2I, SHBG, TRIM63, KDM6A, MYOCD, MKL1, CNTN2, CSNK2A1, SMARCA4, PELP1, KAT5, HDAC4, EPC1, SMAD7, HDAC3, MKL2, ATF6, BARX2, MAPKAPK2, FHL3, GATA4, CAMK2A, HOPX, PYCARD, NKX2-3, MINPP1,
Protein Size: 508
Molecular Weight 52 kDa
Gene ID 6722
NCBI Accession NM_003131, NP_003122
UniProt P11831
Application Notes WB Suggested Anti-SRF Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 508 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
Western Blotting (WB) image for anti-SRF (N-Term) antibody (ABIN2774664) WB Suggested Anti-SRF
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:1562500
Chromatin Immunoprecipitation (ChIP) image for anti-SRF (N-Term) antibody (ABIN2774664) Chromatin Immunoprecipitation (ChIP) Using SRF Antibody - N-terminal region (P100866_...
Background publications Doi, Iso, Yamazaki, Akiyama, Kanai, Sato, Kawai-Kowase, Tanaka, Maeno, Okamoto, Arai, Kedes, Kurabayashi: "HERP1 inhibits myocardin-induced vascular smooth muscle cell differentiation by interfering with SRF binding to CArG box." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 11, pp. 2328-34, 2005 (PubMed).