Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) (Middle Region) antibody

Details for Product No. ABIN2774731, Supplier: Log in to see
Antigen
  • NEDD4-2
  • NEDD4.2
  • RSP5
  • hNEDD4-2
  • nedd4
  • nedd4-2
  • NEDD4
  • Nedd4-2
  • 1300012C07Rik
  • Nedd4b
  • neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
  • NEDD4L
  • neural precursor cell expressed, developmentally down-regulated gene 4-like
  • NEDD4L
  • LOC776799
  • nedd4l
  • Nedd4l
Alternatives
anti-Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like antibody for Western Blotting
Epitope
Middle Region
15
10
9
9
7
3
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
49
47
28
5
4
4
4
4
2
1
1
1
1
Host
Rabbit
57
7
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
43
23
13
7
6
5
4
2
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L
Blocking Peptide Blocking peptide for this product available: ABIN980594
Sequence TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NEDD4L (NEDD4L Antibody Abstract)
Background NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
Protein Size: 955
Molecular Weight 110 kDa
Gene ID 23327
NCBI Accession NM_015277, NP_056092
Research Area Enzymes
Pathways Negative Regulation of Transporter Activity
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 955 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 4-Like (NEDD4L) (Middle Region) antibody (ABIN2774731) WB Suggested Anti-NEDD4L Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Product cited in: Raikwar, Thomas: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, 2008 (PubMed).

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