RAD18 antibody (Middle Region)
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- Target See all RAD18 Antibodies
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAD18 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- KQVTKVDCPV CGVNIPESHI NKHLDSCLSR EEKKESLRSS VHKRKPLPKT
- Predicted Reactivity
- Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against RAD18. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human RAD18
- Top Product
- Discover our top product RAD18 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 495 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, (2008) (PubMed).
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Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, (2008) (PubMed).
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- Target
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Alternative Name
- RAD18 (RAD18 Products)
- Synonyms
- RNF73 antibody, 2810024C04Rik antibody, Rad18sc antibody, zgc:55577 antibody, RAD18 antibody, DDBDRAFT_0218120 antibody, DDBDRAFT_0304878 antibody, DDB_0218120 antibody, DDB_0304878 antibody, DKFZp469H0810 antibody, RAD18, E3 ubiquitin protein ligase antibody, RAD18 E3 ubiquitin protein ligase antibody, E3 ubiquitin-protein ligase RAD18 antibody, E3 ubiquitin-protein ligase antibody, DNA repair protein and ATPase antibody, C3HC4-type zinc finger-containing protein antibody, RAD18 antibody, Rad18 antibody, rad18 antibody
- Background
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RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RNF73
Protein Interaction Partner: TAX1BP1, UBC, SHPRH, RAD18, SIVA1, USP2, UBE2B, UBE2A, HLTF, PCNA, BPLF1, USP5, DBF4, BRCA1, DZIP3, POLH, DTL, Ankrd32, MLKL, ECT2, RPL10A, TRIM23, PCGF6, TRIM8, RFC2, CPD, SPRTN, ZMYM2, MAPK8, POLN, WRNIP1, gag-pol, ORC2, SUMO2, POLK, NBN, Smc6, HIST1H2A
Protein Size: 495 - Molecular Weight
- 56 kDa
- Gene ID
- 56852
- NCBI Accession
- NM_020165, NP_064550
- UniProt
- Q9NS91
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