MCM2 antibody (N-Term)

Catalog No. ABIN2774882

Product Details anti-MCM2 Antibody

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Horse, Cow, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PGRSSRRTDA LTSSPGRDLP PFEDESEGLL GTEGPLEEEE DGEELIGDGM

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MCM2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MCM2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 904 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MCM2 antibody (ABIN2774882)

Rizwani, Alexandrow, Chellappan: "Prohibitin physically interacts with MCM proteins and inhibits mammalian DNA replication." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 10, pp. 1621-9, (2009) (PubMed).

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Synonyms: CG7538 antibody, DmMCM2 antibody, DmMcm2 antibody, DmeMCM2 antibody, Dmel\\CG7538 antibody, MCM2 antibody, MCM2-7 antibody, MCM2_DROME antibody, McM2 antibody, PCR3 antibody, dMCM2 antibody, l(3)rL074 antibody, XMcm2 antibody, bm28 antibody, ccnl1 antibody, cdc19 antibody, cdcl1 antibody, mitotin antibody, cb737 antibody, chunp6905 antibody, BM28 antibody, CCNL1 antibody, CDCL1 antibody, D3S3194 antibody, MITOTIN antibody, MCM7 antibody, AA959861 antibody, AW476101 antibody, Mcmd2 antibody, mKIAA0030 antibody, Minichromosome maintenance 2 antibody, minichromosome maintenance complex component 2 antibody, minichromosome maintenance complex component 2 L homeolog antibody, Mcm2 antibody, mcm2 antibody, MCM2 antibody, mcm2.L antibody

Background: The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Alias Symbols: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN
Protein Interaction Partner: HUWE1, MCM6, CHFR, SUMO2, MCMBP, UBC, SUMO1, MDM2, MCM7, MCM4, HSP90AB1, ACLY, PRRC1, HYOU1, CAP1, NCL, POLA1, TONSL, SNF8, ELL, TCEA1, POLR2A, CDK6, CDK4, CDC6, MCM10, ORC6, CCNB2, VCAM1, P2RX4, ORC5, ORC2, MCM5, MCM3, ITGA4, FN1, CSNK2A1, CDKN2A, DBF4,
Protein Size: 904

Molecular Weight: 99 kDa

Gene ID: 4171

NCBI Accession: NM_004526, NP_004517

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA