UBE2D1 antibody (Middle Region)

Catalog No. ABIN2775671

Product Details anti-UBE2D1 Antibody

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Cow, Goat, Zebrafish (Danio rerio), Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SQWSPALTVS KVLLSICSLL CDPNPDDPLV PDIAQIYKSD KEKYNRHARE

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE2D1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2D1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 147 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2D1

Target: UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))

Alternative Name: UBE2D1 (UBE2D1 Products)

Synonyms: E2(17)KB1 antibody, SFT antibody, UBC4/5 antibody, UBCH5 antibody, UBCH5A antibody, ube2d1 antibody, wu:fc16h06 antibody, zgc:73096 antibody, e2(17)kb1 antibody, sft antibody, ubc4/5 antibody, ubch5 antibody, ubch5a antibody, ubiquitin conjugating enzyme E2 D1 antibody, ubiquitin-conjugating enzyme E2D 1 antibody, ubiquitin-conjugating enzyme E2D 1b antibody, ubiquitin conjugating enzyme E2 D1 S homeolog antibody, UBE2D1 antibody, Ube2d1 antibody, ube2d1b antibody, ube2d1.S antibody, ube2d1 antibody

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Alias Symbols: E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Protein Interaction Partner: RBX1, STUB1, RNF7, RNF14, MID1, MDM2, EP300, CREBBP, ANAPC11, DTL, RNF115, MKRN2, NEDD4L, FAF2, TRIM32, MID2, RNF111, RNF5, RSP5, GPB1, NHLRC1, RFFL, UHRF2, DTX2, ZNRF1, TRIM63, RNF146, FBXO31, RNF26, TRIM39, RNF126, FBXW7, NEDD4, CUL1, CUL3, MKRN3, UBE3A
Protein Size: 147

Molecular Weight: 16 kDa

Gene ID: 7321

NCBI Accession: NM_001204880, NP_003329

UniProt: P51668

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades