Hydroxysteroid (17-Beta) Dehydrogenase 6 Homolog (Mouse) (HSD17B6) (N-Term) antibody

Details for Product No. ABIN2776865, Supplier: Log in to see
Antigen
  • HSE
  • RODH
  • SDR9C6
  • 17betaHSD9
  • Hsd17b9
  • Rdh8
  • hydroxysteroid (17-beta) dehydrogenase 6
  • hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
  • HSD17B6
  • Hsd17b6
Alternatives
anti-Human Hydroxysteroid (17-Beta) Dehydrogenase 6 Homolog (Mouse) antibody for Immunohistochemistry
Epitope
N-Term
5
3
3
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
38
24
21
3
1
1
1
1
1
Host
Rabbit
35
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
23
13
13
11
7
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HSD17B6
Sequence MWLYLAAFVG LYYLLHWYRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against HSD17B6. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name HSD17B6 (HSD17B6 Antibody Abstract)
Background HSD17B6 has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. HSD17B6 is a member of the retinol dehydrogenase family.The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. Transcript variants utilizing alternative polyadenylation signals exist.
Alias Symbols: HSE, RODH, SDR9C6
Protein Size: 317
Molecular Weight 35 kDa
Gene ID 8630
NCBI Accession NM_003725, NP_003716
UniProt O14756
Research Area Signaling, Hormones
Pathways Steroid Hormone Biosynthesis
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 317 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Hydroxysteroid (17-Beta) Dehydrogenase 6 Homolog (Mouse) (HSD17B6) (N-Term) antibody (ABIN2776865) WB Suggested Anti-HSD17B6 Antibody Titration: 1.25ug/ml
Positive Control...
Immunohistochemistry (IHC) image for anti-Hydroxysteroid (17-Beta) Dehydrogenase 6 Homolog (Mouse) (HSD17B6) (N-Term) antibody (ABIN2776865) anti-Hydroxysteroid (17-Beta) Dehydrogenase 6 Homolog (Mouse) (HSD17B6) (N-Term) antibody (Image 2)
Product cited in: Mohler, Titus, Bai, Kennerley, Lih, Tomer, Wilson: "Activation of the androgen receptor by intratumoral bioconversion of androstanediol to dihydrotestosterone in prostate cancer." in: Cancer research, Vol. 71, Issue 4, pp. 1486-96, 2011 (PubMed).

Muthusamy, Andersson, Kim, Butler, Waage, Bergerheim, Gustafsson: "Estrogen receptor ? and 17?-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 50, pp. 20090-4, 2011 (PubMed).

Background publications Huang, Luu-The: "Gene structure, chromosomal localization and analysis of 3-ketosteroid reductase activity of the human 3(alpha-->beta)-hydroxysteroid epimerase." in: Biochimica et biophysica acta, Vol. 1520, Issue 2, pp. 124-30, 2001 (PubMed).

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