Mediator Complex Subunit 6 (MED6) (Middle Region) antibody

Details for Product No. ABIN2778335, Supplier: Log in to see
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
  • RNA polymerase transcriptional regulation mediator-related
  • mediator complex subunit 6
  • mediator of RNA polymerase II transcription, subunit 6 homolog (yeast)
  • RNA polymerase II transcriptional regulation mediator
  • AT3G21350
  • MED6
  • Med6
  • med6
Alternatives
anti-Human Mediator Complex Subunit 6 antibody for Western Blotting
Epitope
Middle Region
15
10
10
9
9
9
9
7
5
2
2
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
74
15
13
6
5
5
4
4
3
3
1
1
1
1
1
Host
Rabbit
61
14
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
71
38
3
3
2
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MED6
Blocking Peptide Blocking peptide for this product available: ABIN979954
Sequence ALLLDLRQKF PPKFVQLKPG EKPVPVDQTK KEAEPIPETV KPEEKETTKN
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against MED6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MED6 (MED6 Antibody Abstract)
Background MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: NY-REN-28
Protein Interaction Partner: TARDBP, UBC, CDK19, CDK8, MED10, MED23, CCNC, MED19, MED26, FBXW7, MED11, MED28, MED29, MED4, MED12, MED7, MED21, MED1, SMARCA4, BCL6, SUZ12, CUL1, SKP1, CTDP1, MED25, SREBF1, MED15, VDR, OBFC1, MED18, ZC3H13, TADA2A, RARA, PARP1, SMAD3, SMAD2, SMAD1, MED
Protein Size: 246
Molecular Weight 28 kDa
Gene ID 10001
NCBI Accession NM_005466, NP_005457
UniProt O75586
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 246 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778335) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Background publications Pavri, Lewis, Kim, Dilworth, Erdjument-Bromage, Tempst, de Murcia, Evans, Chambon, Reinberg: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).