AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody

Details for Product No. ABIN2778782, Supplier: Log in to see
Antigen
  • 5033405N08Rik
  • wu:fb81b10
  • zgc:101057
  • C77140
  • W91705
  • AU RNA binding protein/enoyl-CoA hydratase
  • agmatine ureohydrolase (agmatinase)
  • AU RNA binding protein/enoyl-Coenzyme A hydratase
  • AU RNA binding protein/enoyl-coenzyme A hydratase
  • Auh
  • Agmat
  • auh
  • AUH
Epitope
C-Term
6
5
4
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast
29
17
14
3
2
2
2
1
1
Host
Rabbit
27
4
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
28
14
9
6
Options
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Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human AUH
Blocking Peptide Blocking peptide for this product available: ABIN973655
Sequence IGMSLAKELI FSARVLDGKE AKAVGLISHV LEQNQEGDAA YRKALDLARE
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%
Characteristics This is a rabbit polyclonal antibody against AUH. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name AUH (AUH Antibody Abstract)
Background AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.
Protein Interaction Partner: UBC, AUH,
Protein Size: 339
Molecular Weight 37 kDa
Gene ID 549
NCBI Accession NM_001698, NP_001689
UniProt Q13825
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Metabolism, Organelles
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 339 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782) WB Suggested Anti-AUH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...
Immunohistochemistry (IHC) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782) anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-AU RNA Binding Protein/enoyl-CoA Hydratase (AUH) (C-Term) antibody (ABIN2778782) WB Suggested Anti-AUH
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500
Background publications Illsinger, Lücke, Zschocke, Gibson, Das: "3-methylglutaconic aciduria type I in a boy with fever-associated seizures." in: Pediatric neurology, Vol. 30, Issue 3, pp. 213-5, 2004 (PubMed).