POU1F1 antibody (Middle Region)
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- Target See all POU1F1 Antibodies
- POU1F1 (POU Class 1 Homeobox 1 (POU1F1))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Rabbit, Goat, Sheep, Horse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POU1F1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- KRRTTISIAA KDALERHFGE QNKPSSQEIM RMAEELNLEK EVVRVWFCNR
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 93%, Sheep: 100%
- Characteristics
- This is a rabbit polyclonal antibody against POU1F1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human POU1F1
- Top Product
- Discover our top product POU1F1 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 291 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- POU1F1 (POU Class 1 Homeobox 1 (POU1F1))
- Alternative Name
- POU1F1 (POU1F1 Products)
- Synonyms
- CPHD1 antibody, GHF-1 antibody, PIT1 antibody, POU1F1a antibody, Pit-1 antibody, Hmp1 antibody, Pit1 antibody, Pit1-rs1 antibody, dw antibody, dwarf antibody, GHF1 antibody, GHF1A antibody, PIT1Z antibody, PIT-1 antibody, Pit-1* antibody, pit1 antibody, pit-1 antibody, POU class 1 homeobox 1 antibody, POU domain, class 1, transcription factor 1 antibody, POU1F1 antibody, Pou1f1 antibody, pou1f1 antibody
- Background
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PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP. This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: GHF-1, PIT1, Pit-1, Pit-1 beta, CPHD1, POU1F1a
Protein Interaction Partner: CEBPD, NCOR1, CREBBP, MED1, GATA2, NR1I3, NR1I2, PITX1, JUN, ETS1, NR3C1, VDR, PPARG, PPARA,
Protein Size: 291 - Molecular Weight
- 33 kDa
- Gene ID
- 5449
- NCBI Accession
- NM_000306, NP_000297
- UniProt
- P28069
- Pathways
- Regulation of Carbohydrate Metabolic Process
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