Sirtuin 6 (SIRT6) (Middle Region) antibody

Details for Product No. ABIN2779624, Supplier: Log in to see
Antigen
  • SIR2L6
  • 2810449N18Rik
  • AI043036
  • Sir2l6
  • sirtuin 6
  • sirt6
  • SIRT6
  • Sirt6
Epitope
Middle Region
75
19
19
18
18
16
12
6
6
6
5
5
5
5
5
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Rat (Rattus)
190
71
37
7
4
3
2
2
2
1
1
1
1
Host
Rabbit
187
22
6
Clonality
Polyclonal
Conjugate
Un-conjugated
16
12
10
7
6
6
4
4
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)
179
82
57
32
32
32
14
12
9
4
3
3
2
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human SIRT6
Blocking Peptide Blocking peptide for this product available: ABIN983773
Sequence TRINGSIPAG PKQEPCAQHN GSEPASPKRE RPTSPAPHRP PKRVKAKAVP
Predicted Reactivity Cow: 100%, Dog: 100%, Human: 100%, Rat: 83%
Characteristics This is a rabbit polyclonal antibody against SIRT6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SIRT6 (SIRT6 Antibody Abstract)
Background SIRT6 is a NAD-dependent protein deacetylase. SIRT6 has deacetylase activity towards 'Lys-9' and 'Lys-56' of histone H3. SIRT6 modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates 'Lys-9' of histone H3 at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. SIRT6 may be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. SIRT6 is required for genomic stability, normal IGF1 serum levels and normal glucose homeostasis. SIRT6 modulates cellular senescence and apoptosis. SIRT6 regulates the production of TNF protein.This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Alias Symbols: SIR2L6
Protein Interaction Partner: UBC, MDM2, AKT1, STUB1, SKP2, ABCF2, FAF1, VIM, UBE2D1, CHD3, NR0B2, tat, CCNDBP1, RELA, XRCC5, PRKDC, XRCC6, ELF5,
Protein Size: 355
Molecular Weight 39 kDa
Gene ID 51548
NCBI Accession NM_016539, NP_057623
UniProt Q8N6T7
Research Area Cancer
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 355 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
 image for anti-Sirtuin 6 (SIRT6) (Middle Region) antibody (ABIN2779624) anti-Sirtuin 6 (SIRT6) (Middle Region) antibody
Product cited in: Wajapeyee, Malonia, Palakurthy, Green: "Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors." in: Genes & development, Vol. 27, Issue 20, pp. 2221-6, 2013 (PubMed).

Gazin, Wajapeyee, Gobeil, Virbasius, Green: "An elaborate pathway required for Ras-mediated epigenetic silencing." in: Nature, Vol. 449, Issue 7165, pp. 1073-7, 2007 (PubMed).