AIRE antibody (Autoimmune Regulator) (N-Term)

Details for Product anti-AIRE Antibody No. ABIN2779777, Supplier: Log in to see
Antigen
  • AIRE1
  • APECED
  • APS1
  • APSI
  • PGA1
  • autoimmune regulator
  • autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
  • autoimmune regulator-like
  • AaeL_AAEL013794
  • CpipJ_CPIJ001208
  • CpipJ_CPIJ001209
  • AIRE
  • Aire
  • LOC100621659
Alternatives
anti-Cow (Bovine) AIRE antibody for Western Blotting
Epitope
N-Term
37
18
17
15
10
10
7
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
148
71
29
10
8
7
5
4
3
2
1
Host
Rabbit
96
58
18
1
Clonality
Polyclonal
Conjugate
This AIRE antibody is un-conjugated
8
5
5
5
5
5
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
144
83
39
36
19
18
13
6
5
5
2
1
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AIRE
Blocking Peptide Blocking peptide for this product available: ABIN973041
Sequence HRTEIAVAVD SAFPLLHALA DHDVVPEDKF QETLHLKEKE GCPQAFHALL
Predicted Reactivity Cow: 92%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against AIRE. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name AIRE (AIRE Antibody Abstract)
Background The function of the protein encoded by the AIRE gene is not well defined. However it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Alias Symbols: AIRE1, APECED, APS1, APSI, PGA1
Protein Interaction Partner: TUBB, RAVER1, TUBA1C, TMPRSS13, CCDC87, FAT1, GIGYF2, SRRM2, TUBB4B, EIF4EBP3, UBB, HSP90AB1, HSPA8, HSPA5, HSPA1L, HSPA1A, ATP4A, HIST3H3, HDAC2, HDAC1, DAXX, AIRE, UBAC1, HIST1H3A, HIST2H2BE, HSPA4, SRSF1, SMC1A, XPO1, TOP2A, SSRP1, SNRPD3, SNRPB, SRSF3
Protein Size: 545
Molecular Weight 58 kDa
Gene ID 326
NCBI Accession NM_000383, NP_000374
UniProt O43918
Research Area Immunology, Transcription Factors
Pathways Chromatin Binding
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 545 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Autoimmune Regulator (AIRE) (N-Term) antibody (ABIN2779777) WB Suggested Anti-AIRE Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
 image for anti-Autoimmune Regulator (AIRE) (N-Term) antibody (ABIN2779777) anti-Autoimmune Regulator (AIRE) (N-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Autoimmune Regulator (AIRE) (N-Term) antibody (ABIN2779777) anti-Autoimmune Regulator (AIRE) (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-Autoimmune Regulator (AIRE) (N-Term) antibody (ABIN2779777) AIRE Antibody - N-terminal region (ARP32650_P050) validated by WB using human LCL at ...
Western Blotting (WB) image for anti-Autoimmune Regulator (AIRE) (N-Term) antibody (ABIN2779777) WB Suggested Anti-AIRE
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500 ...
Background publications Ilmarinen, Kangas, Kytömaa, Eskelin, Saharinen, Seeler, Tanhuanpää, Chan, Slattery, Alakurtti, Palvimo, Ulmanen: "Functional interaction of AIRE with PIAS1 in transcriptional regulation." in: Molecular immunology, Vol. 45, Issue 7, pp. 1847-62, 2008 (PubMed).

Akiyoshi, Hatakeyama, Pitkänen, Mouri, Doucas, Kudoh, Tsurugaya, Uchida, Matsushima, Oshikawa, Nakayama, Shimizu, Peterson, Matsumoto: "Subcellular expression of autoimmune regulator is organized in a spatiotemporal manner." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33984-91, 2004 (PubMed).

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