ERCC3 antibody (N-Term)

Catalog No. ABIN2780326

Product Details anti-ERCC3 Antibody

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Human: 100%, Pig: 93%, Rabbit: 79%, Rat: 79%, Yeast: 90%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ERCC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERCC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 782 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ERCC3 antibody (ABIN2780326)

Vijai, Topka, Villano, Ravichandran, Maxwell, Maria, Thomas, Gaddam, Lincoln, Kazzaz, Wenz, Carmi, Schrader, Hart, Lipkin, Neuhausen, Walsh, Zhang, Lejbkowicz, Rennert, Stadler, Robson, Weitzel et al.: "A Recurrent ERCC3 Truncating Mutation Confers Moderate Risk for Breast Cancer. ..." in: Cancer discovery, Vol. 6, Issue 11, pp. 1267-1275, (2016) (PubMed).

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3 (ERCC3 Products)

Synonyms: BTF2 antibody, GTF2H antibody, RAD25 antibody, TFIIH antibody, XPB antibody, BTF2 p89 antibody, Ercc-3 antibody, wu:fc25f08 antibody, ERCC excision repair 3, TFIIH core complex helicase subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 3 antibody, excision repair cross-complementation group 3 antibody, ERCC3 antibody, Ercc3 antibody, ercc3 antibody

Background: ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription.
Alias Symbols: BTF2, GTF2H, RAD25, TFIIH, XPB
Protein Interaction Partner: XIAP, CEP70, UBC, rev, SRPK2, CDK7, TP53, E2F1, CDK8, CCNC, GTF2H5, ERCC2, MSANTD2, UVSSA, POLR2C, KPNA3, RAD52, MMS19, SIX5, GTF2H4, GTF2H3, GTF2H1, tat, COPS2, GTF2H2, NEDD8, ERCC6, POLR2A, AR, CCNH, MYC, MNAT1, ZSCAN1, ATF7IP, GCN1L1, ERCC5, BCR, PSMC5
Protein Size: 782

Molecular Weight: 89 kDa

Gene ID: 2071

NCBI Accession: NM_000122, NP_000113

UniProt: P19447

Pathways: DNA Damage Repair