WT1 antibody (N-Term)

Catalog No. ABIN2780339

Product Details anti-WT1 Antibody

Target: WT1 (Wilms Tumor 1 (WT1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PASQHTLRSG PGCLQQPEQQ GVRDPGGIWA KLGAAEASAE RLQGRRSRGA

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against WT1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 497 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 (WT1 Products)

Synonyms: AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody

Background: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.
Alias Symbols: AWT1, GUD, WAGR, WIT-2, WT33, NPHS4, EWS-WT1
Protein Interaction Partner: KRTAP10-3, KRTAP10-8, KRT40, EGR1, MDM2, DVL3, CIAO1, TP53, HSPA4, TAOK1, NPM3, ZNF205, SUZ12, MEN1, EZH2, DNMT1, WTIP, WTAP, PAWR, UBE2I, TP63, FHL2, TP73, PRKACA, CREBBP, U2AF2, PAX2, AREG,
Protein Size: 497

Molecular Weight: 54 kDa

Gene ID: 7490

NCBI Accession: NM_000378, NP_000369

UniProt: E9PMK7

Pathways: Tube Formation