POLR3A antibody (Middle Region)
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- Target See all POLR3A Antibodies
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig, Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR3A antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT
- Predicted Reactivity
- Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%, Yeast: 85%
- Characteristics
- This is a rabbit polyclonal antibody against POLR3A. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human POLR3A
- Top Product
- Discover our top product POLR3A Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 1390 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
- Alternative Name
- POLR3A (POLR3A Products)
- Synonyms
- 9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
- Background
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Alias Symbols: RPC1, RPC155, hRPC155, ADDH, HLD7
Protein Interaction Partner: SUMO2, UBC, ASB13, NEDD4, POLR3H, VPS18, POLR3B, POLR1D, CRCP, POLR1A, POLR3C, POLR1C, SMARCA5, POLR2H, POLR2E, POLR3D, IKBKG, PCBD1, EWSR1, RPAP2, RPAP3, RPAP1, GPN1, RAE1, POLR3E, PKP2, GTF2B,
Protein Size: 1390 - Molecular Weight
- 156 kDa
- Gene ID
- 11128
- NCBI Accession
- NM_007055, NP_008986
- UniProt
- O14802
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