GNAO1 antibody (Middle Region)

Catalog No. ABIN2786202

Product Details anti-GNAO1 Antibody

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Binding Specificity: Middle Region

Reactivity: Mouse, Human, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Sheep, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: CDVVSRMEDT EPFSAELLSA MMRLWGDSGI QECFNRSREY QLNDSAKYYL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against GNAO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GNAO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 354 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GNAO1 antibody (ABIN2786202)

Zupancic, Blejec, Herman, Veber, Verbovsek, Korsic, Knezevic, Rozman, Turnsek, Gruden, Motaln: "Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach." in: Radiology and oncology, Vol. 48, Issue 3, pp. 257-66, (2014) (PubMed).

Target Details for GNAO1

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Alternative Name: GNAO1 (GNAO1 Products)

Synonyms: G-ALPHA-o antibody, GNAO antibody, AW050213 antibody, Galphao antibody, Gnao antibody, alphaO antibody, gnao1 antibody, wu:fq26h05 antibody, zgc:73315 antibody, RATBPGTPC antibody, XGalpha01 antibody, gnao1-A antibody, zgc:73153 antibody, G protein subunit alpha o1 antibody, guanine nucleotide binding protein, alpha O antibody, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, a antibody, G protein subunit alpha o1 L homeolog antibody, Guanine nucleotide-binding protein G(o) subunit alpha antibody, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, b antibody, GNAO1 antibody, Gnao1 antibody, gnao1a antibody, gnao1.L antibody, goa-1 antibody, gnao1b antibody

Background: Activated Goalpha interacted directly with PLZF, and enhanced its function as a transcriptional and cell growth suppressor. Goalpha might play a role in mediating extracellular signal-regulated kinase activation by G protein-coupled receptors in the brain.
Alias Symbols: DKFZp686O0962, G-ALPHA-o, GNAO
Protein Interaction Partner: UBC, ADRA2A, BAG3, Htt, RGS9, GABBR1, Haus4, RGS1, RIC8A, DCTN2, KLHL3, SCN8A, PDE6G, CRHR1, GPSM2, HRH4, NGB, S1PR5, RGS19, GRM6, FFAR2, TSHR, RGS5, RGS10, RGS7, RGS4, RGS16, RGS14, PTPRU, CCR5, NUCB1, PDE6H, CACNA1B, OPRM1, OPRD1, GNAO1, GNAI1, ADRA2C,
Protein Size: 354

Molecular Weight: 40 kDa

Gene ID: 2775

NCBI Accession: NM_138736, NP_620073

UniProt: B3KP89

Pathways: G-protein mediated Events